Deficiency of mitochondrial fumarase activity in tomato plants impairs photosynthesis via an effect on stomatal function
| Autor: | Adriano Nunes-Nesi, R. George Ratcliffe, James E. Graham, Anna Lytovchenko, Lee J. Sweetlove, Yves Gibon, Joachim Fisahn, Fernando Carrari, Ronan Sulpice, Alisdair R. Fernie |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
2. Zero hunger
0106 biological sciences 0303 health sciences Stomatal conductance biology fungi food and beverages Cell Biology Plant Science 15. Life on land Carbohydrate metabolism biology.organism_classification Photosynthesis 01 natural sciences 03 medical and health sciences Biochemistry Fumarase Botany Genetics Genetically modified tomato Solanum Solanaceae 030304 developmental biology 010606 plant biology & botany Transpiration |
| Zdroj: | The Plant Journal. 50:1093-1106 |
| ISSN: | 0960-7412 |
| DOI: | 10.1111/j.1365-313x.2007.03115.x |
| Popis: | Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of a fumarate hydratase (fumarase) gene in the antisense orientation and exhibiting considerable reductions in the mitochondrial activity of this enzyme show impaired photosynthesis. The rate of the tricarboxylic acid cycle was reduced in the transformants relative to the other major pathways of carbohydrate oxidation and the plants were characterized by a restricted rate of dark respiration. However, biochemical analyses revealed relatively little alteration in leaf metabolism as a consequence of reducing the fumarase activity. That said, in comparison to wild-type plants, CO 2 assimilation was reduced by up to 50% under atmospheric conditions and plants were characterized by a reduced biomass on a whole plant basis. Analysis of further photosynthetic parameters revealed that there was little difference in pigment content in the transformants but that the rate of transpiration and stomatal conductance was markedly reduced. Analysis of the response of the rate of photosynthesis to variation in the concentration of CO 2 confirmed that this restriction was due to a deficiency in stomatal function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text