Monitoring Single S. cerevisiae Cells with Multifrequency Electrical Impedance Spectroscopy in an Electrode-Integrated Microfluidic Device
| Autor: | Yangye Geng, Zhen Zhu, Yingying Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Materials science
biology business.industry 010401 analytical chemistry Microfluidics Saccharomyces cerevisiae 02 engineering and technology 021001 nanoscience & nanotechnology Hydrodynamic trapping biology.organism_classification 01 natural sciences Multiplexing 0104 chemical sciences Microelectrode Single-cell analysis Electrode Optoelectronics 0210 nano-technology business Electrical impedance spectroscopy |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071608210 |
| DOI: | 10.1007/978-1-0716-0822-7_9 |
| Popis: | This chapter describes an electrode-integrated microfluidic system with multiple functions of manipulating and monitoring single S. cerevisiae cells. In this system, hydrodynamic trapping and negative dielectrophoretic (nDEP) releasing of S. cerevisiae cells are implemented, providing a flexible method for single-cell manipulation. The multiplexing microelectrodes also enable sensitive electrical impedance spectroscopy (EIS) to discern the number of immobilized cells, classify different orientations of captured cells, as well as detect potential movements of immobilized single yeast cells during the overall recording duration by using principal component analysis (PCA) in data mining. The multifrequency EIS measurements can, therefore, obtain sufficient information of S. cerevisiae cells at single-cell level. |
| Databáze: | OpenAIRE |
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