Action of human interleukin‐4 and stem cell factor on erythroid and mixed colony formation by peripheral blood‐derived CD34 + c‐kit high or CD34 + c‐kit low cells

Autor: Hideaki Sakabe, Takafumi Kimura, Steven C. Clark, Shouhei Yokota, Tatsuo Abe, Yoshiaki Sonoda, Yoshikazu Ohmizono, Shigeatsu Tanimukai
Rok vydání: 1997
Předmět:
Zdroj: British Journal of Haematology. 96:781-789
ISSN: 1365-2141
0007-1048
DOI: 10.1046/j.1365-2141.1997.d01-2091.x
Popis: We studied the interaction of interleukin (IL)-4 and other burst-promoting activity (BPA) factors, such as IL-3, granulocyte/macrophage colony-stimulating factor (GM-CSF), IL-9 and stem cell factor (SCF), on erythroid burst-forming unit (BFU-E) and erythrocyte-containing mixed (CFU-Mix) colony formation in serum-free culture. IL-4 alone did not support mixed colony formation in the presence of erythropoietin (Epo). However, IL-4 showed weak but significant BPA when peripheral blood (PB)-derived CD34 + c-kit low cells were used as the target population. The BPA of IL-4 was much weaker than that of IL-3, which exerted the most potent activity, as previously reported. When CD34 + c-kit high cells were used as the target, four factors known to have BPA, IL-3, GM-CSF, IL-9 and SCF, could express BPA. In contrast, IL-4 alone failed to support erythroid burst formation. Interestingly, IL-4 showed a remarkable enhancing effect with SCF in promoting the development of erythroid burst and erythrocyte-containing mixed colonies from CD34 + c-kit low and CD34+c-kit high cells. Delayed addition of SCF+Epo or IL-4+Epo to the cultures initiated with either IL-4 or SCF alone clearly demonstrated that SCF was a survival factor for both BFU-E and CFU-Mix progenitors. In contrast, the survival effect of IL-4 was much weaker than that of SCF, and appeared to be more important for progenitors derived from CD34 + c-kit low cells than for those derived from CD34 + c-kit high cells. It was recently reported that CD34 + c-kit low cells represent a more primitive population than CD34 + c-kit high cells. Taken together, these results suggest that IL-4 helps to recruit primitive progenitor cells in the presence of SCF.
Databáze: OpenAIRE
Externí odkaz: