Effect of ethylene on Agrobacterium tumefaciens-mediated gene transfer to melon

Autor:	Tsuyoshi Yasuta, Hiroshi Ezura, Ken-Ichi Yuhashi, Kiwamu Minamisawa
Rok vydání:	2000
Předmět:	Reporter gene Ethylene biology Agrobacterium Melon Genetic transfer food and beverages Plant Science Agrobacterium tumefaciens biology.organism_classification chemistry.chemical_compound Biochemistry chemistry Gene expression Genetics Agronomy and Crop Science Explant culture
Zdroj:	Plant Breeding. 119:75-79
ISSN:	1439-0523 0179-9541
DOI:	10.1046/j.1439-0523.2000.00438.x
Popis:	The effect of ethylene on gene transfer mediated by an Agrobacterium tumefaciens harbouring a binary vector with the beta-glucuronidase (uidA) gene was investigated in melon, Cucumis melo L. Explants excised from melon cotyledons produced ethylene, the production of which was increased by the addition of 1-aminocyclopropane-1-carboxylic acid (ACC, 20 or 200 micromolar), and inhibited by the addition of aminoethoxy-vinylglycin (AVG, 10 micromolar). Agrobacterium inoculation of explants increased ethylene production, while application of AVG during inoculation reduced it. After 4 days of co-cultivation with Agrobacterium, gene transfer in the explants was assayed by transient uidA expression. Application of ACC to the co-cultivation medium reduced Agrobacterium-mediated gene transfer to explants and that of AVG increased it. These results suggest that ethylene affects the A. tumefaciens-mediated gene transfer to the explants excised from melon cotyledons, and the efficiency of Agrobacterium-mediated gene transfer can be improved by inhibiting ethylene production from the explants.
Databáze:	OpenAIRE
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