Covalent Functionalization for Biomolecular Recognition on Vertically Aligned Carbon Nanofibers

Autor:	Beth M. Nichols, Kiu-Yuen Tse, Sarah E. Baker, and Tami Lasseter Clare, Eve Hindin, Robert J. Hamers
Rok vydání:	2005
Předmět:	chemistry.chemical_classification Materials science Oligonucleotide Carbon nanofiber General Chemical Engineering Biomolecule General Chemistry Glassy carbon Combinatorial chemistry chemistry Covalent bond Nanofiber Materials Chemistry Organic chemistry Molecule Amine gas treating
Zdroj:	Chemistry of Materials. 17:4971-4978
ISSN:	1520-5002 0897-4756
DOI:	10.1021/cm051024d
Popis:	We compare two different strategies for covalently modifying carbon nanofibers with biological molecules such as DNA. One method begins with a photochemical reaction between the nanofibers and molecules bearing both a terminal olefin group and a protected amine group followed by deprotection to yield the free primary amine. The second method uses a chemical reaction of an aryldiazonium salt with the nanofibers followed by electrochemical reduction to the primary amine. Both methods then link the primary amines to thio-terminated DNA oligonucleotides. Our measurements show that both methods yield DNA-modified carbon nanofibers exhibiting excellent specificity and reversibility in binding to DNA probe molecules in solution having complementary vs noncomplementary sequences. Quantitative measurements show that 2.3 × 1014 DNA molecules/cm2 will hybridize to the DNA-modified nanofiber samples, approximately eight times higher than for a flat sample of glassy carbon functionalized in an identical manner. Simila...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69a58ce621fa08715563461bc3d86548 https://doi.org/10.1021/cm051024d Zobrazit plný text záznamu