Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis: in vitro and in vivo assessment
Autor: | Eric C. Reynolds, Neil M O'Brien-Simpson, Wayne A. Morrison, Thomas J. Webster, Andrea J. O'Connor, Phong A. Tran, Nathalie Bock, Anand K. Deva, Jason A. Palmer |
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Rok vydání: | 2019 |
Předmět: |
Biophysics
Pharmaceutical Science Bioengineering 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences Microbiology Biomaterials Antibiotic resistance Staphylococcus epidermidis Intensive care Drug Discovery Bone plate medicine biology business.industry Organic Chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Antimicrobial Methicillin-resistant Staphylococcus aureus 0104 chemical sciences Staphylococcus aureus Implant 0210 nano-technology business |
Zdroj: | International Journal of Nanomedicine. 14:4613-4624 |
ISSN: | 1178-2013 |
Popis: | Background: Bacterial infection is a common and serious complication in orthopedic implants following traumatic injury, which is often associated with extensive soft tissue damage and contaminated wounds. Multidrug-resistant bacteria have been found in these infected wounds, especially in patients who have multi trauma and prolonged stay in intensive care units.Purpose: The objective of this study was to develop a coating on orthopedic implants that is effective against drug-resistant bacteria. Methods and results: We applied nanoparticles (30-70nm) of the trace element selenium (Se) as a coating through surface-induced nucleation-deposition on titanium implants and investigated the antimicrobial activity against drug resistant bacteria including Methicillin-resistant Staphylococcus aureus (MRSA) and Methicillin-resistant Staphylococcus epidermidis (MRSE) in vitro and in an infected femur model in rats.The nanoparticles were shown in vitro to have antimicrobial activity at concentrations as low as 0.5ppm. The nanoparticle coatings strongly inhibited biofilm formation on the implants and reduced the number of viable bacteria in the surrounding tissue following inoculation of implants with biofilm forming doses of bacteria. Conclusion: This study shows a proof of concept for a selenium nanoparticle coatings as a potential anti-infective barrier for orthopedic medical devices in the setting of contamination with multi-resistant bacteria. It also represents one of the few (if only) in vivo assessment of selenium nanoparticle coatings on reducing antibiotic-resistant orthopedic implant infections. |
Databáze: | OpenAIRE |
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