NMR-based Structural Classification, Identification, and Quantification of Triterpenoids from Edible Mushroom Ganoderma resinaceum

Autor:	Xing-Rong Peng, Guilin Hu, Hai-Guo Su, Yan-Jie Huang, Ming-Hua Qiu, Jian-Chao Chen, Adelakun Tiwalade Adegoke, Gang Wei, Xian Li
Rok vydání:	2020
Předmět:	Edible mushroom Chromatography Triterpenoid biology Ganoderma Chemistry Absolute quantification Ganoderma resinaceum General Chemistry Structural classification General Agricultural and Biological Sciences biology.organism_classification Two-dimensional nuclear magnetic resonance spectroscopy
Zdroj:	Journal of Agricultural and Food Chemistry. 68:2816-2825
ISSN:	1520-5118 0021-8561
DOI:	10.1021/acs.jafc.9b07791
Popis:	Ganoderma mushrooms have been widely used as functional food in China, Japan, and Korea. Ganoderma triterpenoids are deemed to be the main functional constituents. The structures of Ganoderma triterpenoids are complex but quite similar, which makes their analyses markedly limited. In this study, we developed a general 2D NMR method to differentiate Ganoderma triterpenoids, which classifies them into six types (A-F). Then, by the NMR-based isolation of A-F type triterpenoids from the fruiting bodies of G. resinaceum, four new compounds (1-4) and eight known compounds (5-12) were obtained. Moreover, combined with spiking experiments in 1D and 2D NMR spectra, compounds 5, 7, and 8, which belong to triterpenoids of A and B types, were identified. At the end, to achieve a more extensive application for this NMR method, a qNMR method for the absolute quantification of 5, 7, and 8 in the gross triterpenoids from G. resinaceum was set up. The results showed that this NMR method is reliable for the NMR-guided isolation and quantification of triterpenoids in G. resinaceum.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::699bb0bc476796fe7e4d70d45ef5b895 https://doi.org/10.1021/acs.jafc.9b07791 Zobrazit plný text záznamu