METHOD FOR IMPLEMENTING BIOLUMINESCENCE-BASED ANALYTICAL ASSAYS IN NANOLITER VOLUMES

Autor: Jd Andrade, Rh Davies, Da Bartholomeusz, Tsm Yang
Rok vydání: 2005
Předmět:
Zdroj: Bioluminescence and Chemiluminescence.
DOI: 10.1142/9789812702203_0054
Popis: INTRODUCTION Bioluminescence-based analytical assays were used to measure various analytes in nanoliter sample volumes. Nanoliter volumes of multiple bioluminescent analytical assays were deposited in an array format and lyophilized. ATP-firefly luciferase (FFL) and NADH-bacterial luciferase (BL) platform reactions were compared. We achieved parallel sample delivery via sample-hydrated membranes. A CCD camera measured the luminescent kinetics for each assay. These miniaturized assays and instruments can be prepared as micro-analytical systems to operate in point-of-care (POC) diagnostic devices.
Databáze: OpenAIRE