Radial Immunodiffusion: a Simple and Rapid Method for Detection of Marek's Disease Antigen(s)
| Autor: | W. W. Marquardt |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Radial immunodiffusion
Antiserum Marek's disease animal structures General Immunology and Microbiology General Medicine Biology Precipitin biology.organism_classification Virology General Biochemistry Genetics and Molecular Biology Immunodiffusion Antigen Feather visual_art visual_art.visual_art_medium Flock General Pharmacology Toxicology and Pharmaceutics |
| Zdroj: | Applied Microbiology. 23:942-945 |
| ISSN: | 0003-6919 |
| DOI: | 10.1128/am.23.5.942-945.1972 |
| Popis: | A qualitative radial immunodiffusion technique is described which detects antigen(s) in feathers from live or dead chickens infected with Marek's disease herpesvirus. Antiserum, which is incorporated into a support medium, reacts with antigen(s) in the feather tip producing a radial precipitin ring. Antigen(s) was detected in 93.3% of experimentally inoculated chickens 21 days postinoculation and in 100% of infected birds subsequently tested through 6 weeks. No antigen was detectable in the feathers of uninoculated control chickens. The technique is simple and rapid to perform. Positive tests could be detected after 1 to 2 hours of incubation. Antigen detection by the radial immunodiffusion test correlated well with other criteria of infection. This technique should have application as a laboratory research tool and as an adjunct for a rapid flock diagnosis of Marek's disease. |
| Databáze: | OpenAIRE |
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