Identification of embryogenic microspores of barley (Hordeum vulgare L.) by individual selection and culture and their potential for transformation by microinjection

Autor:	H. U. Koop, Mechthild Bolik
Rok vydání:	1991
Předmět:	Microculture Lucifer yellow Stamen food and beverages Cell Biology Plant Science General Medicine Biology chemistry.chemical_compound Transformation (genetics) Tissue culture Microspore chemistry Botany Hordeum vulgare Microinjection
Zdroj:	Protoplasma. 162:61-68
ISSN:	1615-6102 0033-183X
Popis:	In order to identify microspores, suitable for transformation via microinjection of DNA, single microspores of barley (Hordeum vulgare L.) were selected after initial preculture of anthers floating on liquid media and analysed for their development in individual culture in microdroplets of culture medium. Conditions for microculture and plant regeneration from single selected embryogenie microspores were established. The technical feasability of intranuclear microinjection was demonstrated by injecting the fluorescent dye Lucifer Yellow. All essential procedures for a transformation system of barley based on microinjection into microspores have thus been performed successfully. Further efforts to increase efficiencies of culture and microinjection procedures are necessary, however, in order to improve the suitability of this approach towards transformation of barley.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::697b204c64a7d283f4db05e591b66f26 https://doi.org/10.1007/bf01403902 Zobrazit plný text záznamu Full text from SpringerLink