Comparison of Conjugase Treatment Procedures in the Microbiological Assay for Food Folacin

Autor:	A. J. Kirsch, Tung-Shan Chen
Rok vydání:	1984
Předmět:	Chromatography biology Microbiological assay Spinach biology.organism_classification Hog kidney Food Science Incubation period
Zdroj:	Journal of Food Science. 49:94-98
ISSN:	1750-3841 0022-1147
DOI:	10.1111/j.1365-2621.1984.tb13678.x
Popis:	Variations in the conjugase treatment step of the microbiological assay for food folacin were investigated. The source of the conjugase–fresh hog kidney, fresh chicken pancreas or commercial dried chicken pancreas–did not significantly affect the measurable folacin activity of spinach. However, the buffer, pH, incubation time, sequence of steps in extraction and conjugase treatment, and method of sample filtration (filter paper vs centrifuge) used prior to conjugase treatment were all found to significantly affect the folacin activity of spinach.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6964c9aa30fb9cf1a7b7af93bd27e38e https://doi.org/10.1111/j.1365-2621.1984.tb13678.x Zobrazit plný text záznamu Plný text