Preliminary Comparative Assessment of Brucellergene Skin Test for Diagnosis of Brucellosis in Dromedary Camels (Camelus dromedarius)

Autor:	Fabrizio De Massis, Abdelghani Benkhelil, Mohamed A Al Hosani, Armando Giovannini, Abdelmalik I. Khalafalla, Rashid Khan, Paolo Calistri, Salama S. Al Muhairi, Javed Rashid, Khalafallah M Alamin, Irfan A Khan
Rok vydání:	2020
Předmět:	Veterinary medicine Infectious Diseases Abu dhabi Specific test business.industry Virology medicine Brucellosis Skin test medicine.disease business Microbiology Serology
Zdroj:	Vector-Borne and Zoonotic Diseases. 20:412-417
ISSN:	1557-7759 1530-3667
DOI:	10.1089/vbz.2019.2537
Popis:	This study was conducted to evaluate the use of Brucellergene skin test (BST) for the diagnosis of Brucellosis in camels (Camelus dromedarius) in comparison with Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 68 apparently healthy adult dromedary camels of either gender from three different geographical locations of Abu Dhabi Emirate, United Arab Emirates (UAE), were included in the study. The skin test was applied on two shaved areas at the middle of the neck: one for the test and the other area was injected with normal saline as a control. Reading was done 72 h postinjection. Results were subjected to Bayesian analysis to assess the test performances in camels. The model estimated the following sensitivity and specificity median values: BST: Se = 70.72%, Sp = 98.82%; RBT: Se = 93.27%, Sp = 97.79%; and c-ELISA: Se = 94.78%, Sp = 98.48%. As the BST investigated in this study proved to be a highly specific test, we propose using it as a confirmatory test in camels particularly when the serological tests give doubtful results on individual animals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69643968ded495edd245fccdec67d422 https://doi.org/10.1089/vbz.2019.2537 Zobrazit plný text záznamu