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Bacteria of the genus Bacillus are ubiquitous in nature and produce several antimicrobial compounds, being increasingly used in plant biocontrol. The objective of this study was to identify the bacterium isolate Bacillus sp. 1.4 at the species level and study its bioactive properties prospecting a potential in agricultural application. The bacterial isolate was identified as belonging to Bacillus altitudinis by 16S rRNA gene sequence analysis and genomic metrics. The antimicrobial substance extracted with butanol inhibited Listeria monocytogenes ATCC 7644 and Bradyrhizobium japonicum CT 00345 with inhibition halos of 12 and 13 mm, respectively. In the exopolysaccharide production assay, B. altitudinis1.4 presented a negative result and in the evaluation of motility through the swarming assay, 90 mm halos were observed in both agar concentrations (0.3 and 0.7%) for up to 72 h of incubation. Genomic analysis revealed genes potentially coding for features that might both be beneficial for plants and improve the competition of B. altitudinis 1.4 in soil. This bacterium was able to solubilize inorganic phosphate, coexist with B. japonicum CT 00345, and form more biofilm when grown together. Based on the results found and with new tests to be performed, it is suggested that the B. altitudinis 1.4 isolate could be a candidate for plant growth promoter. |
