Application of SPE for the HPLC analysis of taxanes fromTaxus cell cultures
Autor: | Robert Verpoorte, Georgios Theodoridis, C. F. de Jong, Gregory Laskaris |
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Rok vydání: | 1998 |
Předmět: | |
Zdroj: | Chromatographia. 47:25-34 |
ISSN: | 1612-1112 0009-5893 |
DOI: | 10.1007/bf02466782 |
Popis: | Solid phase extraction (SPE) methods are studied for the sample pretreatment for HPLC analysis ofTaxus cell suspension cultures. Various types of SPE materials were tested for the extraction of both the taxane standards and samples of various origin. Comparison between the different cartridges and the different elution solvents are made in terms of extraction recovery and sample clean-up. Selective elution of the taxanes is achieved by a gradient elution scheme. |
Databáze: | OpenAIRE |
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