Abstract 62: Dietary Fibre Attenuates Hypertension By Reducing Macrophage Infiltration Via Gut Microbiota-Derived Metabolite-Sensing Receptors
| Autor: | Joanne O'Donnell, Christopher K. Barlow, Charles R. Mackay, Maria Kaparakis-Liaskos, Rikeish R. Muralitharan, Michael E Nakai, Evany Dinakis, Chudan Xu, Madeleine Paterson, Ekaterina Salimova, Natalie J. Bitto, Francine Z Marques, Darren Creek, Hamdi A. Jama, Liang Xie |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Hypertension. 78 |
| ISSN: | 1524-4563 0194-911X |
| Popis: | High dietary fibre is fermented by the gut microbiota, resulting in the release of metabolites called short-chain fatty acids (SCFAs). Both fibre and SCFAs can reduce high blood pressure (BP) and its associated cardio-renal complications. However, the underlying mechanisms remain unclear. SCFAs can be detected by metabolite-sensing receptors GPR41 and GPR43, highly expressed by immune cells such as macrophages. We hypothesised that dietary fibre attenuates hypertension by modulating renal macrophage infiltration via metabolite-sensing receptors GPR41 and GPR43. To test this, we developed a novel GPR41/GPR43 double knockout (DKO) mice and characterised the cardiovascular and immune phenotype in both sham and angiotensin-II (Ang-II, 0.5mg/kg/day) treated DKO and wild-type (WT) mice (n=7-12 per group). WT Ang-II mice fed a high-fibre diet had significantly lower renal galectin-3 (p=0.0004), a macrophage marker, compared to WT Ang-II mice fed a low-fibre diet. Sham DKO mice on standard chow diet had no difference in BP or heart function but had higher kidney/tibia length index (p=0.049) and renal fibrosis levels compared to WT mice (p=0.004). Moreover, untreated DKO mice had higher numbers of renal macrophages compared to WT mice (p=0.002). Ang-II infusion of DKO mice resulted in higher BP (p |
| Databáze: | OpenAIRE |
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