Comparison of sensitization pattern to dust mite allergens between atopic dermatitis patients and dogs and non-specific reactivity of canine IgE to storage mite, Tyrophagus putrescentiae

Autor: Hangyeol Song, Jongsun Lee, Kyoung Yong Jeong, Doo-Sung Cheon, Jung-Won Park
Rok vydání: 2022
DOI: 10.21203/rs.3.rs-1852939/v1
Popis: House dust mite is common cause of atopic dermatitis (AD) both in human and dogs. Detection of serum IgE to allergen is commonly used to diagnose allergic diseases. However, false-positive reactions due to cross-reactivity and non-specific reactivity may lead to misdiagnosis. We compared human and canine IgE reactivities to mite component allergens. Canine IgE-reactive components of D. farinae and T. putrescentiae were identified by tandem mass spectrometry. Recombinant proteins were produced and IgE reactivities to component allergens were assessed by ELISA and inhibition assays using sera from AD patients and dogs. Canine IgE-reactive proteins (Der f 1, Der f 11, Tyr p 4, Tyr p 8, Tyr p 11, Tyr p 28) were identified by proteome analysis. The majority of patients were sensitized to Der f 1 (93.3%) and Der f 2 (86.7%). Dogs showed high sensitization to Der f 2 (94.1%) and Der f 18 (84.6%). Both patients and dogs showed low IgE binding frequency to Tyr p 8, 43.3% and 4% respectively. The ELISA inhibition study indicated that canine IgE reactivity to T. putrescentiae is mostly due to non-specific reaction and cross-reaction with D. farinae. Different IgE sensitization patterns were shown between allergic human and dogs with AD, especially to Der f 18 in Korea for the first time. Furthermore, non-specific canine IgE reactivity to storage mite indicates the possible misdiagnoses. Standardizations focused on the major canine allergen content of extracts should be developed. This will allow precision diagnosis and individuated treatments for each patient and atopic dog.
Databáze: OpenAIRE
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