Human papillomavirus DNA in cervix

Autor: F. Bejui-Thivolet, N. Liagre, Yvette Chardonnet, M.C. Chignol, L. M. Patricot
Rok vydání: 1992
Předmět:
Zdroj: Pathology - Research and Practice. 188:67-73
ISSN: 0344-0338
Popis: Summary We examined retrospectively a series of 65 Bouin's fixed, paraffin-embedded tissue specimens from 8 condylomatous lesions, 16 condylomas associated with cervical intraepithelial neoplasia (CIN), and 12 neoplasia without condylomatous signs, for histological characteristics, the detection of viral structural antigen, the presence and typing of HPV DNA by molecular in situ hybridization with biotinylated probes types 6, 11,16 and 18 under stringent conditions (Tm -12 °C. HPV DNA was present in 34165 (52%) specimens. Detection of viral structural antigen was positive in only 14% (3122) specimens. HPV DNA were identified in 919 (100%) condylomatous lesions (with HPV type 6, 11, 18). Three condylomas were coin fected with both HP V type 6 or 11 and type 18; viral antigen was found in two specimens. HPV DNA were detected in 18131 (58%) low grade and advanced CIN associated with condylomatous changes (type 6 = 5 specimens, type 11 = 3 specimens, type 16 = 4 specimens, type 18 = 6 specimens). Four of these cases were coin fected with both HPV type 6/11 and HPV type 16118. Viral antigen was negative in all specimens. HPV DNA were detected in 7125 (28%) advanced infra-cervical neoplasia (CIN III) without anatomopathological condylomatous changes (type 6 - 1 specimen, type 16 = 3 specimens, type 18 = 3 specimens). One of these specimens contained both HP V types 6 and 18. Viral antigen was found in one case. Our data confirm the association of HP V types 6 and 11 with condyloma and low grade neoplasia; HP V types 16 and 18 were associated with advanced cervical neoplasia. Our results point up the frequency of coin fection with different HPV DNA 6 or 11 and 16 or 18 in the same lesion. The detection of potentially oncogenic HPV types in condylomas without cervical neoplasia is of interest since these patients are at high risk of developing malignancy. In situ hybridization with blotinylated probes is a useful, sensitive and appropriate method for retrospective analysis of HPV DNA sequences even in routinely Bouin's fixed, , paraffin -embedded cervical lesions. In some special clinical cases this technique may be useful to identify patients at. risk of more serious or possibly malignant progression.
Databáze: OpenAIRE
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