Evaluation of jatropha oil to produce poly(3-hydroxybutyrate) by Cupriavidus necator H16
| Autor: | Wei-Yang Ooi, Ko-Sin Ng, R. Shenbagarathai, Lay-Koon Goh, Kumar Sudesh |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
Polymers and Plastics biology Linoleic acid Cupriavidus necator Jatropha Condensed Matter Physics biology.organism_classification Palmitic acid chemistry.chemical_compound Oleic acid Vegetable oil chemistry Dry weight Mechanics of Materials Materials Chemistry biology.protein Organic chemistry Food science Lipase |
| Zdroj: | Polymer Degradation and Stability. 95:1365-1369 |
| ISSN: | 0141-3910 |
| DOI: | 10.1016/j.polymdegradstab.2010.01.021 |
| Popis: | Jatropha oil, a non-edible vegetable oil, may be an alternative substrate to food-grade oils for bioplastic production. Jatropha oil contains 93.9% palmitic acids, oleic acids and linoleic acids. High P(3HB) accumulation of 87 wt% from 13.1 g/L of cell dry weight (CDW) was obtained by Cupriavidus necator H16 when 12.5 g/L of jatropha oil and 0.54 g/L of urea were used. Lipase activity increased in the initial stages of P(3HB) production, when 1 g/L of jatropha oil was added to the preculture medium. Addition of oil in preculture did not affect final CDW or P(3HB) accumulation. P(3HB) production in a 10 L lab-scale fermenter gave a yield of 0.78 g P(3HB) per g jatropha oil used after 48 h. For the first time, this study proved that jatropha oil is a feasible and excellent carbon source for P(3HB) biosynthesis by C. necator H16 with potential for large-scale production. The toxins in jatropha oil did not affect the P(3HB) biosynthesis. |
| Databáze: | OpenAIRE |
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