Intravenous xenogeneic transplantation of human adipose-derived stem cells improves left ventricular function and microvascular integrity in swine myocardial infarction model
| Autor: | Brian H. Johnstone, Chris Bull, Peng Sheng Chen, Jessica Warfel, Stephanie Merfeld-Clauss, Aaron M. Kyle, Keith L. March, Dongni Feng, Maja Deuter-Reinhard, Jie Xie, Jonathan R. Lindner, Dmitry O. Traktuev, John Kihlken, Soon Jun Hong, Pamela I. Rogers |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Ejection fraction Ventricular function business.industry Adipose tissue Coronary flow reserve General Medicine medicine.disease Surgery Internal medicine medicine Cardiology Radiology Nuclear Medicine and imaging Myocardial infarction Stem cell Cardiology and Cardiovascular Medicine business Perfusion CD8 |
| Zdroj: | Catheterization and Cardiovascular Interventions. 86:E38-E48 |
| ISSN: | 1522-1946 |
| Popis: | Objectives The potential for beneficial effects of adipose-derived stem cells (ASCs) on myocardial perfusion and left ventricular dysfunction in myocardial ischemia (MI) has not been tested following intravenous delivery. Methods Surviving pigs following induction of MI were randomly assigned to 1 of 3 different groups: the placebo group (n = 7), the single bolus group (SB) (n = 7, 15 × 107 ASCs), or the divided dose group (DD) (n = 7, 5 × 107 ASCs/day for three consecutive days). Myocardial perfusion defect area and coronary flow reserve (CFR) were compared during the 28-day follow-up. Also, serial changes in the absolute number of circulating CD4+T and CD8+T cells were measured. Results The increases in ejection fraction were significantly greater in both the SB and the DD groups compared to the placebo group (5.4 ± 0.9%, 3.7 ± 0.7%, and −0.4 ± 0.6%, respectively), and the decrease in the perfusion defect area was significantly greater in the SB group than the placebo group (−36.3 ± 1.8 and −11.5 ± 2.8). CFR increased to a greater degree in the SB and the DD groups than in the placebo group (0.9 ± 0.2, 0.8 ± 0.1, and 0.2 ± 0.2, respectively). The circulating number of CD8+T cells was significantly greater in the SB and DD groups than the placebo group at day 7 (3,687 ± 317/µL, 3,454 ± 787/µL, and 1,928 ± 457/µL, respectively). The numbers of small vessels were significantly greater in the SB and the DD groups than the placebo group in the peri-infarct area. Conclusions Both intravenous SB and DD delivery of ASCs are effective modalities for the treatment of MI in swine. Intravenous delivery of ASCs, with its immunomodulatory and angiogenic effects, is an attractive noninvasive approach for myocardial rescue. © 2014 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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