α-Lactalbumin and the Lactose Synthetase Reaction

Autor:	I. Kiyosawa, B. Colvin, R. Mawal, D.K. Fitzgerald, K.E. Ebner, Urs Brodbeck
Rok vydání:	1970
Předmět:	Lactalbumin Physiological function animal structures biology Chemistry Kinetics Lactose Synthetase Cell Biology Biochemistry chemistry.chemical_compound Galactose Alpha-lactalbumin biology.protein Lactose Molecular Biology Uracil nucleotide
Zdroj:	Journal of Biological Chemistry. 245:2103-2108
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(18)63212-0
Popis:	An improved procedure for the isolation of the A protein of lactose synthetase is presented. The Km for UDP-galactose is not influenced by the concentration of glucose or α-lactalbumin but these compounds alter the maximum velocity of the reaction. There is a reciprocal relationship between the concentration of glucose and α-lactalbumin in the lactose synthetase assay and α-lactalbumin lowers the apparent Km of glucose. It is suggested that the physiological function of α-lactalbumin is to lower the Km of glucose so that it may be used maximally for the synthesis of lactose. Lactose may be synthesized at maximum rates by the A protein in the absence of α-lactalbumin but in the presence of high concentrations of glucose. Different preparations of the A protein have variable activities.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6918ae5bf03b280811185c37e8d834e4 https://doi.org/10.1016/s0021-9258(18)63212-0 Zobrazit plný text záznamu