Construction of SEC/CTC electrodes for direct electron transferring biosensors
| Autor: | Golam Faruque Khan |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
biology
Immobilized enzyme Stereochemistry Chemistry Inorganic chemistry Metals and Alloys Enzyme electrode Condensed Matter Physics Polypyrrole Surfaces Coatings and Films Electronic Optical and Magnetic Materials Crystal Electron transfer chemistry.chemical_compound Electrode Materials Chemistry biology.protein Glucose oxidase Electrical and Electronic Engineering Instrumentation Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 36:484-490 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/s0925-4005(97)80117-7 |
| Popis: | A stable organic charge-transfer-complex (CTC) electrode for the direct oxidation of flavoproteins has been constructed and the mode of electron transfer is described. Tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) is grown at the surface of a shapable electroconductive (SEC) film (a polyanion-doped polypyrrole film) in such a way that it makes a tree-shaped crystal structure standing vertically on the surface. Glucose oxidase (GOD) is adsorbed and cross-linked under dry conditions with glutaraldehyde to fix direct electron transfer between the active center of the enzyme and the crystal electrode takes place, which leads to a glucose sensor with remarkably improved performance. The kinetic parameters of the GOD/CTC/SEC electrode are reported. A mechanism of direct electron transfer is proposed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect