Development and growth of plantlets ofPinus contortaregenerated from adventitious buds
| Autor: | Karl-Anders Högberg, Roland Grönroos, Gunnar Flygh, Sara von Arnold |
|---|---|
| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Scandinavian Journal of Forest Research. 13:331-339 |
| ISSN: | 1651-1891 0282-7581 |
| DOI: | 10.1080/02827589809382992 |
| Popis: | Before micropropagation techniques can be applied to a particular species, it is crucial to optimize the method and to determine how the micropropagated plants grow in the field. Adventitious shoots developed on embryos of Pinus contorta Dougl. ex Loud, after a 2 h pulse treatment with 250 μM N6‐benzyladenine. The time to first subculture after the pulse treatment influenced the yield of adventitious shoots. On average, 68% of the adventitious shoots had developed roots 12 weeks after treatment with 1.25 mM indole‐3‐butyric acid for 6 h. The auxin treatment stimulated early rooting (i.e. within 6 weeks) but had no effect on late rooting (i.e. after 6 weeks). The size of the plantlets was of importance for their survival when potted. All plantlets with a distinct stem elongated during the first growth period while some without did not. The relative height growth rate of plantlets was similar to that of seedlings. In the field the increase of height was similar for plantlets and seedlings. Plagiotropy was h... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|