The bcl-2 knockout mouse exhibits marked changes in osteoblast phenotype and collagen deposition in bone as well as a mild growth plate phenotype

Autor:	Theologos M. Michaelidis, J. Denton, Gillian A. Wallis, M. C. Hillarby, Raymond P. Boot-Handford, A. Zambelli, Michael E. Grant, Judith A. Hoyland, Anthony J. Freemont
Rok vydání:	2002
Předmět:	Pathology medicine.medical_specialty Ossification Dwarfism Osteoblast Cell Biology Biology medicine.disease Phenotype Pathology and Forensic Medicine Cell biology medicine.anatomical_structure In vivo Osteoclast Knockout mouse medicine medicine.symptom Molecular Biology Endochondral ossification
Zdroj:	International Journal of Experimental Pathology. 79:329-335
ISSN:	0959-9673
DOI:	10.1046/j.1365-2613.1998.790411.x
Popis:	Histological examination of long bones from 1-day-old bcl-2 knockout and age-matched control mice revealed no obvious differences in length of bone, growth plate architecture or stage of endochondral ossification. In 35-day-old bcl-2 knockout mice that are growth retarded or 'dwarfed'. the proliferative zone of the growth plate appeared slightly thinner and the secondary centres of ossification less well developed than their age-matched wild-type controls. The most marked histological effects of bcl-2 ablation were on osteoblasts and bone. 35-day-old knockout mouse bones exhibited far greater numbers of osteoblasts than controls and the osteoblasts had a cuboidal phenotype in comparison with the normal flattened cell appearance. In addition, the collagen deposited by the osteoblasts in the bcl-2 knockout mouse bone was disorganized in comparison with control tissue and had a pseudo-woven appearance. The results suggest an important role for Bcl-2 in controlling osteoblast phenotype and bone deposition in vivo.
Databáze:	OpenAIRE
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