Optimization of decolorization process in agar production from Gracilaria lemaneiformis and evaluation of antioxidant activities of the extract rich in natural pigments
Autor: | Yingnian Lu, Zhihong Duan, Xiaoli Ma, Sheng Wang, Shengliang Yuan |
---|---|
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Antioxidant food.ingredient Chemistry DPPH medicine.medical_treatment Extraction (chemistry) Environmental Science (miscellaneous) Raw material Agricultural and Biological Sciences (miscellaneous) 03 medical and health sciences Pigment chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine food Functional food visual_art 030221 ophthalmology & optometry visual_art.visual_art_medium medicine Agar Hydroxyl radical Food science Biotechnology |
Zdroj: | 3 Biotech. 8 |
ISSN: | 2190-5738 2190-572X |
DOI: | 10.1007/s13205-017-1037-6 |
Popis: | Gracilaria lemaneiformis is mainly used as a raw material source for agar industry, and its extract is rich in natural pigments with antioxidant activities. In this study, a solvent reflux extraction method for decolorization of G. lemaneiformis has been developed in agar production. The extraction conditions were optimized as follows: solvent-to-material ratio, 50:1; ethanol concentration, 70%; number of extractions, 3; extraction time, 0.5 h, under which the total antioxidant yield of the extract reached 2.89 ± 0.88 mg/g dried seaweeds. Their IC50 values of DPPH radical scavenging activity, hydroxyl radical scavenging activity and superoxide anion scavenging activity were 21.91 ± 1.8 mg/L, 40.59 ± 1.5 mg/L and 160.87 ± 2.8 mg/L, respectively. Further isolation and spectroscopic analysis of natural pigments suggested the strong antioxidant capacities were attributed to chlorophyll derivatives. The results indicate that the decolorization process was able to improve the agar quality, and the extract containing lots of natural pigments had antioxidant activities which may be used in functional food and cosmetics. |
Databáze: | OpenAIRE |
Externí odkaz: |