Involvement ofEscherichia coliK-12 recombination functions in elimination of multicopy plasmid R6K by DNA-damaging agents

Autor:	P.V. Attfield, R.J. Pinney
Rok vydání:	1985
Předmět:	RecBCD DNA damage DNA repair Mutant Wild type Biology medicine.disease_cause Microbiology Molecular biology Plasmid Genetics medicine biology.protein bacteria DNA polymerase I Molecular Biology Escherichia coli
Zdroj:	FEMS Microbiology Letters. 28:287-291
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1985.tb00807.x
Popis:	Drugs that damage DNA by different mechanisms eliminate an F- lac + plasmid and multicopy plasmid R6K from Escherichia coli K-12 wild type ( polA + rec + ). However, low-copy plasmid R1 is refractory to elimination under identical conditions. R6K is not eliminated from a polA mutant, which suggests that curing is not due to reduced availability of DNA polymerase I for plasmid replication in cells undergoing DNA repair. R6K is eliminated almost three times faster from a recB recC sbcB mutant than from wild-type strains, whereas elimination from a recF mutant occurs at a rate similar to wild type. Hence, recombination may be involved in drug-mediated elimination of R6K, particularly in cells that carry out recF -dependent recombination exclusively.
Databáze:	OpenAIRE
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