Transverse Dynamic Force Spectroscopy: A Novel Approach to Determining the Complex Stiffness of a Single Molecule
| Autor: | Terence J McMaster, Massimo Antognozzi, Andrew D. L. Humphris, Mervyn J Miles |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Microscope Chemistry Force spectroscopy Stiffness Nanotechnology Surfaces and Interfaces Polymer Condensed Matter Physics Molecular physics law.invention Optical tweezers law Electrochemistry medicine Force dynamics Dissipative system Molecule General Materials Science medicine.symptom Spectroscopy |
| Zdroj: | Langmuir. 18:1729-1733 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Piconewton force sensitivity and angstrom position control offered by instruments such as atomic force microscopy (AFM) and optical tweezers have enabled the force−extension response of single molecules to be investigated. However, to fully investigate the dynamic and energetic properties of a single molecule, it is necessary to detect both conservative (elastic) and dissipative (viscous) components of the extension response. We present a transverse dynamic force microscope (TDFM) capable of measuring this complex quantity. This new force spectroscopy technique offers true control over the tip−surface distance revealing information not accessible by conventional dc atomic force spectroscopy. Results are presented for the force extension response of a single polysaccharide molecule. To the authors knowledge, this is the first time the complex mechanical properties of a single polymer molecule have been measured by TDFM. These observations are in agreement with previous dynamic AFM experiments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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