| Autor: |
Yang Wang, Chengjian Han, Rongsheng Zhou, Jinjin Zhu, Famin Zhang, Jingyang Li, Luo Qingli, Jian Du, Deyong Chu, Yihong Cai, Jilong Shen, Li Yu |
| Rok vydání: |
2021 |
| DOI: |
10.21203/rs.3.rs-155820/v1 |
| Popis: |
The predominant genotype of T. gondii in China is Chinese 1 (ToxoDB#9) lineage. TgCtwh3 and TgCtwh6 are two representative strains of Chinese 1, exhibiting high virulence and low virulence to mice, respectively. Little is known about the virulence mechanism of this non classical genotype. Our previous RNA sequencing data revealed that differential mRNA level of TgMIC1 in TgCtwh3 and TgCtwh6. To further confirm the differential expression of TgMIC1 and its significance in this atypical genotype, quantitative real-time PCR was used to verify the RNA sequencing data firstly, and then polyclonal antibodies against TgMIC1 were prepared and identified. Moreover, the invasion and proliferation of the parasite in HFF cells were observed after treatment with TgMIC1 polyclonal antibody or not. The data showed the protein level of TgMIC1 was significantly higher in high virulence strain TgCtwh3 than that in low virulence strain TgCtwh6, and the invasion and proliferation of TgCtwh3 were inhibited by TgMIC1 polyclonal antibody. Differential expression of TgMIC1 in TgCtwh3 and TgCtwh6 may explain, at least partly, the virulence mechanism of this atypical genotype |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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