Protective role of edaravone against neomycin-induced ototoxicity in zebrafish

Autor:	June Choi, Ah Young Chung, Seung Hoon Lee, Sung Won Chae, Hae Chul Park, Hak Hyun Jung, Gi Jung Im, Jiwon Chang, Hyung Jin Jun, Soon Young Kwon
Rok vydání:	2013
Předmět:	TUNEL assay Anatomy Neomycin Biology Toxicology medicine.disease biology.organism_classification Molecular biology chemistry.chemical_compound Dose–response relationship medicine.anatomical_structure Ototoxicity chemistry Apoptosis medicine Edaravone Hair cell Zebrafish medicine.drug
Zdroj:	Journal of Applied Toxicology. 34:554-561
ISSN:	0260-437X
Popis:	Aminoglycosides such as neomycin are one of the most commonly prescribed types of antibiotics worldwide. However, these drugs appear to generate free radicals within the inner ear, which can result in permanent hearing loss. We evaluated the effects of edaravone, a neuroprotective agent, on neomycin-induced ototoxicity in transgenic zebrafish. The 5-day post fertilization (dpf) zebrafish larvae were exposed to 125 μM neomycin and various concentrations of edaravone for 1 h. Hair cell survival was calculated as average numbers of the hair cells in the control group, which was not exposed to neomycin. Ultrastructural changes were evaluated using a scanning electron microscope (SEM) and transmission electron microscope (TEM). Edaravone protected against neomycin-induced hair cell loss in the neuromasts (1000 μM: 11.6 ± 1.1 cells, neomycin only: 5.5 ± 0.5 cells; n = 10, P
Databáze:	OpenAIRE
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