HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC STEM BARK EXTRACT OF KNEMA ATTENUATA (HOO K. F. AND THOMSON) WARB

Autor:	H Supriya Raja
Rok vydání:	2020
Předmět:	Pharmacology Antioxidant biology medicine.medical_treatment Pharmaceutical Science Glutathione biology.organism_classification Malondialdehyde chemistry.chemical_compound chemistry Toxicity Carbon tetrachloride medicine MTT assay Viability assay Knema attenuata
Zdroj:	International Journal of Pharmacy and Pharmaceutical Sciences. :78-82
ISSN:	0975-1491 2656-0097
Popis:	Objective: To investigate the hepatoprotective activity of ethanolic stem bark extract (ESBE) of Knema attenuata against carbon tetrachloride (CCl4) induced hepatotoxicity in Wistar rats using both in vivo and in vitro models. Methods: Animals were treated orally with ESBE (250 mg kg-1 and 500 mg kg-1) once daily for 6 d and CCl4 on the 4th d. On the 7th d, animals were sacrificed and the blood samples were collected to measure the serum levels of biochemical parameters, whereas the liver homogenates were utilized for estimating the antioxidant defense. The hepatoprotective efficacy of the extract was further ensured in vitro using human liver hepatocellular carcinoma (HepG2) cell line against CCl4 induced toxicity. The cell line viability was determined using 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Results: ESBE effectively reduced (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::68b8933a15b41f4a752513fcfbffe33f https://doi.org/10.22159/ijpps.2020v12i9.38172 Zobrazit plný text záznamu