Degradation of sodium anthraquinone sulphonate by free and immobilized bacterial cultures

Autor: Marc Deront, Meriem Bokhamy, Chantal Seignez, N. Adler, Paul Péringer, Cesar Pulgarin
Rok vydání: 1994
Předmět:
Zdroj: Applied Microbiology and Biotechnology. 41:110-116
ISSN: 1432-0614
0175-7598
DOI: 10.1007/bf00166091
Popis: Aerobic biodegradation of a xenobiotic recalcitrant compound sodium anthraquinone-2-sulphonate (SAS), was investigated using as an inoculum a mixed microbial culture, which was activated sludge from industrial and domestic waste-water treatment plants. The difference in SAS degradation was examined using two main systems: (1) suspended cells and (2) immobilized cells, both in batch and in continuous culture. In the suspended cell system, under continuous culture conditions using SAS as a unique source of carbon and energy, it was possible to degrade about 95% of this substrate after 6 days. Maximal SAS removal rates in the suspended-cell system were 593 mg SAS l−1 h−1 and 88.7 mg SAS l−1 h−1 for dilution rates (D) of 0.05 h−1 and 0.075 h−1, respectively. In the immobilized-cell system, almost all SAS was degraded in 6 days and the maximal removal rate reached 88.7 mg SAS l−1 h−1 at D=0.05 h−1. Application of a continuous-flow enrichment procedure resulted in selection of several kinds of micro-organisms and led to a progressive elimination of some species of Aeromonas. A stable microbial community of 11 strains has been established and characterized at D=0.075 h−1. Most of them were Gram-negative and belonged to the genus Pseudomonas.
Databáze: OpenAIRE
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