Autor:	Jan Wery, Jan C. Verdoes, A.J.J. van Ooyen
Rok vydání:	1998
Předmět:	Plasmid preparation Transposable element Transformation (genetics) Plasmid Terminator (genetics) Electroporation Biology Applied Microbiology and Biotechnology Biochemistry Molecular biology Yeast Transformation efficiency
Zdroj:	Biotechnology Techniques. 12:399-405
ISSN:	0951-208X
Popis:	An efficient transformation system for the astaxanthin-producing yeast Phaffia rhodozyma was developed based on electroporation that routinely yields approximately 1000 transformants per μg of plasmid DNA. The high transformation efficiency depends on vector integration in the ribosomal DNA (rDNA) and the presence of the homologous glycolytic glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter and terminator to drive the expression of the transposon Tn5 encoded kanamycin resistance gene (KmR) as a selective marker. Using this system stable transformants were obtained, carrying multiple plasmid copies. Plasmid copy number could be markedly increased by deletion of the gpd terminator from the transforming plasmid.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6895db5f2760c2c5eb11e4f2b9eaff69 https://doi.org/10.1023/a:1008834600770 Zobrazit plný text záznamu Full text from SpringerLink