Sterilization of Ocular Medical Inserts with Immobilized Proteins
| Autor: | S. S. Dekina, M. V. Balashova, I. I. Romanovskaya, A. M. Ovsepyan |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Pharmaceutical Chemistry Journal. 49:275-279 |
| ISSN: | 1573-9031 0091-150X |
| DOI: | 10.1007/s11094-015-1269-x |
| Popis: | The influence of sterilization of ocular medicinal inserts with human serum albumin (HSA), lysozyme, papain, and urea on the preservation of their physicochemical properties was studied. It was found that the physicochemical properties of inserts with HSA and lysozyme preserved totally their physicochemical properties after sterilization by γ-irradiation in doses of 10, 17, and 28 kGy. The influence of γ-irradiation on the proteolytic activity of ocular medicinal inserts with papain and urea was dose-dependent and reduced the activity by 85 and 93% and led to its complete loss. Adding antiseptics used in the production of ophthalmologic preparations (nipagin, sodium metabisulfite, benzyl alcohol, benzalkonium chloride, etc.) caused an activity loss of the ocular medicinal inserts with papain and urea and did not provide microbiological purity and sterility. UV irradiation of ocular medicinal inserts with papain and urea produced microbiologically pure inserts that preserved 63.8% of the proteolytic activity. Manufacturing of ocular medicinal inserts with papain and urea under aseptic conditions using sterilizing filtration (79.7% activity preservation) was recommended for sterilization. |
| Databáze: | OpenAIRE |
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