The role of 2-C-Methylerythritol-2,4-cyclopyrophosphate in the resuscitation of the 'nonculturable' forms of Mycobacterium smegmatis

Autor:	Arseny S. Kaprelyants, J. Wiesner, Elena G. Salina, A. V. Goncharenko, A. A. Sandanov, Yu. V. Ershov, D. N. Ostrovsky, G. N. Vostroknutova
Rok vydání:	2007
Předmět:	chemistry.chemical_classification biology Transition (genetics) Kinase Mycobacterium smegmatis Bacterial genome size biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Enzyme Biosynthesis chemistry Gene Bacteria
Zdroj:	Microbiology. 76:147-152
ISSN:	1608-3237 0026-2617
DOI:	10.1134/s0026261707020038
Popis:	2-C-Methyl-D-erythritol-2,4-cyclopyrophosphate (MEC), an intermediate of the biosynthesis of isoprenoid compounds in bacteria, was found to be capable of exerting a resuscitating effect on resting Mycobacterium smegmatis cells. The introduction of an additional copy of the ispE gene encoding cytidyl-methyl-erythritol kinase, an enzyme involved in MEC synthesis in M. smegmatis, resulted in the emergence of a capacity for spontaneous reactivation of “nonculturable” M. smegmatis cells, which is not characteristic of the wild-type cells of this species. The involvement of MEC in the transition from the “nonculturable” state to the state of active growth is indicative of a previously unknown function of MEC, assumed to consist in regulation of the bacterial genome activity.
Databáze:	OpenAIRE
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