Highly effective production of biologically active, secreted, human granulocyte-macrophage colony-stimulating factor by recombinant vaccinia virus
| Autor: | V. A. Rikhter, G. V. Kochneva, Elena V. Kuligina, A. V. Tkacheva, G. F. Sivolobova, I. P. Gileva, A. A. Grazhdantseva |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Messenger RNA viruses Biology Recombinant virus Applied Microbiology and Biotechnology Biochemistry Virology Molecular biology Virus law.invention Oncolytic virus 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Granulocyte macrophage colony-stimulating factor chemistry law 030220 oncology & carcinogenesis Recombinant DNA medicine Vaccinia Gene medicine.drug |
| Zdroj: | Applied Biochemistry and Microbiology. 52:685-691 |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | The recombinant vaccinia virus strain VV-GMCSF-S1/3, which contains an insertion of full-length DNA copy of messenger RNA of human granulocyte-macrophage colony-stimulating factor (GM-CSF) in the structural part of the viral thymidine kinase gene, was obtained. The expression of the GM-CSF gene as a part of the recombinant virus is under the control of the native vaccinia virus promoter р7.5K; this results in the production of a mature form of the secreted protein with a molecular mass of 32 kDa. The biological activity of GM-CSF was evaluated by stimulation of the proliferation of cytokine-dependent human TF-1 erythroleukemia cells. The secretion level of biologically active human GM-CSF in the system of recombinant vaccinia virus/mammalian cells was 1–40 μg/mL of culture medium. The recombinant strain VV-GMCSF-S1/3 can be used as a producer of the glycosylated mature form of human GM-CSF, as well as a vector for the construction of oncolytic viruses and multivalent vaccine preparations. |
| Databáze: | OpenAIRE |
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