Sex determination of bovine embryos using the polymerase chain reaction

Autor:	J. R. Miller, M. Schwerin, Mette Schmidt, Birthe Avery, A. Schröder, K. Roschlau, P. H. Poulsen, Preben D. Thomsen
Rok vydání:	1990
Předmět:	Preimplantation Embryos Bioengineering Karyotype Embryo In situ hybridization Biology Molecular biology law.invention chemistry.chemical_compound chemistry law embryonic structures Animal Science and Zoology Bovine embryo Primer (molecular biology) Polymerase chain reaction DNA Biotechnology
Zdroj:	Animal Biotechnology. 1:121-133
ISSN:	1532-2378 1049-5398
DOI:	10.1080/10495399009525735
Popis:	One or two cell biopsies were obtained from 6‐7 days old bovine embryos. The sex of the embryos was determined with two different bovine Y‐chromosome‐specific primer pairs by using the polymerase chain reaction. These results were confirmed by karyotyping as well as in situ hybridization with an independent bovine Y‐chromosome‐specific sequence. The polymerase chain reaction was found to be a quick and accurate method of sex diagnosis of bovine preimplantation embryos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::685ffcd156512366c11908261bb6c3a0 https://doi.org/10.1080/10495399009525735 Zobrazit plný text záznamu