Fibroblast viability and inhibitory activity againstPseudomonas aeruginosain lactic acid-grafted chitosan hydrogels
| Autor: | Lenin Tamay de Dios, Andres Espadín, Cristina Velasquillo, Keiko Shirai, Miquel Gimeno, Nadia Vázquez, Alberto Tecante |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Materials science Polymers and Plastics Biocompatibility General Chemistry Sulfonic acid Surfaces Coatings and Films Lactic acid Chitosan chemistry.chemical_compound chemistry Chitin Self-healing hydrogels Materials Chemistry Organic chemistry Viability assay Lactic acid fermentation Nuclear chemistry |
| Zdroj: | Journal of Applied Polymer Science. 131 |
| ISSN: | 0021-8995 |
| Popis: | Chitosan (Ch) from biologically obtained chitin by lactic acid fermentation process was successfully grafted with lactic acid following three different procedures, direct D,L-lactic acid attachment with and without the aid of p-toluene sulfonic acid catalyst and by ring-opening of L-lactide. The produced materials behave as hydrogels responsive to pH changes and were characterized by X- ray diffraction analyses, swelling behavior, erosion, contact angle, and thermally. In addition, mechanical studies in films of the syn- thetized materials demonstrate that the increase in grafting improved the mechanical properties in terms of tensile strengths. All the grafted Chs showed inhibition of the pathogenic bacteria Pseudomonas aeruginosa, which corroborate the preservation of antimicro- bial activities in the modified Ch. The study on cell attachment and viability of fibroblasts onto the grafted Ch films evidenced that regardless of the low mechanical improvement in the lowest incorporation of lactic acid (31%), it rendered the materials with the highest adherence and viability of fibroblasts. Nonetheless, lactic acid incorporation enhanced cell viability in all synthetized materials as compared to native Ch. V C 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40252. |
| Databáze: | OpenAIRE |
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