Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms
| Autor: | Zoran Jaglic, Dana Cervinkova, Vladimir Babak, Kamila Kyrova, Elleni Michu |
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| Rok vydání: | 2011 |
| Předmět: |
Micrococcaceae
biology Sodium Biofilm food and beverages Pasteurization chemistry.chemical_element biochemical phenomena metabolism and nutrition Carbohydrate medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology law.invention Microbiology fluids and secretions chemistry law Staphylococcus epidermidis medicine Staphylococcus Bacteria Food Science |
| Zdroj: | International Dairy Journal. 21:179-184 |
| ISSN: | 0958-6946 |
| Popis: | Staphylococcus epidermidis may form biofilms in milk processing plants and secondarily contaminate milk products. In this study, the ability of S. epidermidis to form a biofilm in milk and the influence of glucose and NaCl on biofilm formation were analysed. Biofilm production on stainless steel was investigated at 30 °C in pasteurized milk and in milk containing glucose, NaCl or both glucose and NaCl. Biofilm formation was evaluated by monitoring of colony-forming units (cfu) on stainless chips and by real-time quantitative PCR analysis of the ica A and σ B transcripts. An increase in cfu and ica A transcription was observed after 20 h incubation, especially in milk containing glucose and NaCl. NaCl alone was shown to present a greater risk of biofilm development in milk than glucose alone, but the NaCl/glucose combination represented the highest risk. The σ B factor did not seem to be involved in biofilm formation under the conditions of this study. |
| Databáze: | OpenAIRE |
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