A new assembly pathway for the cytokinetic Z ring from a dynamic helical structure in vegetatively growing cells of Bacillus subtilis
| Autor: | Phoebe C. Peters, Elizabeth J. Harry, Carola Thoni, Margaret D. Migocki |
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| Rok vydání: | 2007 |
| Předmět: |
macromolecular substances
Bacillus subtilis Cell cycle Biology Ring (chemistry) biology.organism_classification physiological processes Microbiology Transport protein Cell biology Protein structure biology.protein bacteria biological phenomena cell phenomena and immunity FtsA FtsZ Molecular Biology Cytokinesis |
| Zdroj: | Molecular Microbiology. 64:487-499 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.2007.05673.x |
| Popis: | The earliest event in bacterial cell division is the formation of a Z ring, composed of the tubulin-like FtsZ protein, at the division site at midcell. This ring then recruits several other division proteins and together they drive the formation of a division septum between two replicated chromosomes. Here we show that, in addition to forming a cytokinetic ring, FtsZ localizes in a helical-like pattern in vegetatively growing cells of Bacillus subtilis. FtsZ moves rapidly within this helix-like structure. Examination of FtsZ localization in individual live cells undergoing a single cell cycle suggests a new assembly mechanism for Z ring formation that involves a cell cycle-mediated multistep remodelling of FtsZ polymers. Our observations suggest that initially FtsZ localizes in a helical pattern, with movement of FtsZ within this structure occurring along the entire length of the cell. Next, movement of FtsZ in a helical-like pattern is restricted to a central region of the cell. Finally the FtsZ ring forms precisely at midcell. We further show that another division protein, FtsA, shown to interact with FtsZ prior to Z ring formation in B. subtilis, also localizes to similar helical patterns in vegetatively growing cells. |
| Databáze: | OpenAIRE |
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