Molecular verification of the integration ofTripsacum dactyloides DNA into wheat genome through wide hybridization

Autor:	Chuanyou Li, Jianhang Jia, Demin Jin, Bin Wang, Jiwen Qiu, Ping Ouyang, Dawei Li, William Tai
Rok vydání:	2000
Předmět:	Genetics Multidisciplinary Operon food and beverages Biology biology.organism_classification Genome RAPD chemistry.chemical_compound Molecular level chemistry Tripsacum dactyloides Botany Doubled haploidy Restriction fragment length polymorphism DNA
Zdroj:	Chinese Science Bulletin. 45:528-532
ISSN:	1861-9541 1001-6538
DOI:	10.1007/bf02887099
Popis:	RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=42) and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=72) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloides (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level. This idea has been further testified by RFLP analysis. Application and potentials of transferring Tripsacum dactyloides DNA into wheat genome by sexual hybridization in wheat breeding are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6838871b883ae7c900bce398e69f6eb9 https://doi.org/10.1007/bf02887099 Zobrazit plný text záznamu Full text from SpringerLink