Viability of Gyr (Bos indicus) in vitro produced embryos after vitrification under field conditions
| Autor: | José Carlos Ferreira-Silva, Lucas Carvalho Pereira, Joaquim Corrêa Andrade, Marcos Antonio Lemos Oliveira, Bartira Pastor de Andrade Souza, Humberto Fernandes Veloso Neto, Marcelo Tigre Moura, Ludymila Furtado Cantanhêde |
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| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Boletim de Indústria Animal. 73:159-164 |
| ISSN: | 1981-4100 0067-9615 |
| DOI: | 10.17523/bia.v73n2p159 |
| Popis: | The aim of this study was to evaluate the pregnancy rate of in vitro produced embryos of Gyr cattle ( Bos indicus ) after cryopreservation by the vitrification method under field conditions. Blastocysts in different developmental stages were transferred to recipient cows either fresh (n = 140) or warmed after vitrification (n = 138). The pregnancy rates obtained for fresh embryos were 46.15% (initial blastocyst), 46.93% (blastocyst) and 50.0% (expanded blastocyst) at 35 days post-fertilization, and 43.58% (initial blastocyst), 46.93% (blastocyst) and 50.0% (expanded blastocyst) at 60 days. The pregnancy rates after embryo vitrification were 35.0% (initial blastocyst), 42.3% (blastocyst) and 43.47% (expanded blastocyst) at 35 days post-fertilization, and 32.5% (initial blastocyst), 38.46% (blastocyst) and 43.47% (expanded blastocyst) at 60 days. Embryo vitrification or blastocyst development stage did not affect pregnancy rates or the incidence of embryonic death. In conclusion, vitrification of Gyr ( Bos indicus) embryos under field conditions is an efficient method that can be implemented to use surplus in vitro produced embryos without affecting pregnancy rates. |
| Databáze: | OpenAIRE |
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