Investigation of in vitro anti-arthritic activity of aqueous extracts of leaves of Vitex negundo L. using methotrexate as DMARDs
| Autor: | Sandeep B Patil, Deepak G Joshi, Rakesh Kumar Jat |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Pharmacognosy and Phytochemistry. 10:963-965 |
| ISSN: | 2349-8234 2278-4136 |
| DOI: | 10.22271/phyto.2021.v10.i2m.13926 |
| Popis: | The aim of the study was to investigate the aqueous extracts of Vitex negundo L. for its in vitro anti-arthritic activity by protein denaturation inhibition assay and membrane stabilization method. Methotrexate is one of the most popular and effective drug used worldwide for the treatment of inflammatory conditions like rheumatoid arthritis. The main cause of inflammation in rheumatoid arthritis is protein denaturation. Production of auto antigen in certain rheumatic disease was important for inflammation as well as arthritis. HRBC membrane stabilization was similar to lysosomal membrane which influences the process of inflammation. Methotrexate was used as a standard drug. The percentage of protein denaturation and membrane stabilization for aqueous extracts were done at different concentrations (100, 200, 400, 800, 1000 µg/ml). The maximum inhibition of protein denaturation and membrane stabilization of aqueous extracts of Vitex negundo was found to be 65.62 ±1.10 and 71.87 ±1.46 at dose of 1000 µg/ml respectively and standard inhibition of protein denaturation and membrane stabilization using methotrexate was found to be 87.50±7.02 and 81.25±3.51at 100 µg/ml respectively. The aqueous extracts of Vitex negundo L. showed significant activity at the highest concentration. |
| Databáze: | OpenAIRE |
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