| Autor: |
E Franco, Vicente Mirabet, Javier Monleon, Pilar Solves, M. Guillen, Rosa Moraga, Luis Larrea, Soler Ma, Dolores Planelles, F. C. Uberos, Ana Andrés, T. Planells, Eric Saucedo |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Acta Obstetricia et Gynecologica Scandinavica. 82:439-442 |
| ISSN: |
0001-6349 |
| DOI: |
10.1034/j.1600-0412.2003.00129.x |
| Popis: |
Background and objectives. Collection strategy is the first step for collecting good quality cord blood (CB) units. There are two principal different techniques to collect CB from the umbilical vein: in the delivery room while the placenta is still in the uterus by midwives and obstetricians or in an adjacent room after placental delivery by CB-bank trained personnel. In this study, the benefits and disadvantages between two different CB collection strategies were evaluated in order to improve CB bank methodology. Design and methods. Valencia CB bank maintains the two different collection strategies aforementioned. Before processing CB units, volume was calculated and samples were drawn for cell counts. After processing and before cryopreservation, samples for cell counts, CD34 analysis, viability, clonogenic assays and microbiology were drawn directly from the bags. We compared the efficiency of the two collection techniques. Results. Obstetric date and umbilical CB was obtained from 848 vaginal (484 col... |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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