The inhibitory effects of probiotics on colon cancer cells: in vitro and in vivo studies
| Autor: | Zengren Zhao, Dongyun Li, Haobo Wang, Shihao Chen, Shang Guo, Xia Jiang, Weifang Yu, Fangjian Shang, Ying Liu, Xin Wang, Guiqi Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Colorectal cancer business.industry Gastroenterology Spleen medicine.disease In vitro Metastasis 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Immune system Oncology In vivo Apoptosis 030220 oncology & carcinogenesis Cancer research medicine business CD8 |
| Zdroj: | Journal of Gastrointestinal Oncology. 11:1224-1232 |
| ISSN: | 2219-679X 2078-6891 |
| DOI: | 10.21037/jgo-20-573 |
| Popis: | Background Colorectal cancer (CRC) is the most common gastrointestinal malignancy. And probiotics may have the function of preventing colon cancer. The aim of this study was to investigate the inhibitory effects of a probiotic mixture on colorectal cancer and its potential mechanisms. Methods The effects of the probiotic mixture on proliferation and metastasis of mouse colon cancer CT26 cells were assessed by probiotics and cells co-culture assay, Cell Counting Kit-8 assay, colony formation assay, wound-healing assay, as well as migration and invasion assays. And CT26 cells were also transplanted into BALB/c mice to construct transplanted tumor animal model. The mice were randomly divided into two groups, control group and probiotic mixture intragastric administration group, after injection 21 days the tumor size and infiltration of immune cells in the tumor or spleen tissues were analyzed by hematoxylin and eosin (HE) and immunohistochemistry (IHC) staining. Results The probiotic mixture significantly inhibited the proliferation, invasion, and migration ability of CT26 cells compare to the control cells (P |
| Databáze: | OpenAIRE |
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