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Betel quid chewing is a detrimental recreational habit amongst Asians and a risk factor for oral cancer. Arecoline, a component of areca nut (a major constituent of betel quid) is a known carcinogen. However, the effect of areca nut crude extract is not much studied. To evaluate the cytotoxicity and morphologic effects of areca nut aqueous extract on mouse fibroblast cell line (L929). Dried raw areca nut obtained from a local market in Kota Bharu, Kelantan was prepared and suspended in DMEM (Dulbecco’s Modified Eagle’s medium), prior to serial dilution of 1.56, 0.781, 0.39, 0.195, 0.0976, 0.0488, and 0.0244 mg/ml. The L929 was then exposed to each of the aqueous areca nut extract dilutions and incubated at 37 °C for 24, 48 and 72 hours. Following incubation, the cytotoxicity level of treated L929 was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] assay. Five highest concentrations of areca nut extract showed significantly decreased L929 cell viability (1.56, 0.781, 0.39, 0.195, 0.0976 mg/ml) for all incubation periods compared to untreated cells (p |
