Glucose limitation andpka1deletion rescue aberrant mitotic spindle formation induced by Mal3 overexpression inSchizosaccharomyces pombe
| Autor: | Makoto Kawamukai, Takuma Tanabe, Yasuhiro Matsuo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Chemistry Organic Chemistry Mutant General Medicine biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Spindle apparatus Cell biology Chromosome segregation 03 medical and health sciences 030104 developmental biology Microtubule Glycogenesis Schizosaccharomyces pombe Protein kinase A Molecular Biology Multipolar spindles Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 84:1667-1680 |
| ISSN: | 1347-6947 0916-8451 |
| Popis: | The cAMP-dependent protein kinase Pka1 is known as a regulator of glycogenesis, transition into meiosis, proper chromosome segregation, and stress responses in Schizosaccharomyces pombe. We demonstrated that both the cAMP/PKA pathway and glucose limitation play roles in appropriate spindle formation. Overexpression of Mal3 (1–308), an EB1 family protein, caused growth defects, increased 4C DNA content, and induced monopolar spindle formation. Overproduction of a high-affinity microtubule binding mutant (Q89R) and a recombinant protein possessing the CH and EB1 domains (1–241) both resulted in more severe phenotypes than Mal3 (1–308). Loss of functional Pka1 and glucose limitation rescued the phenotypes of Mal3-overexpressing cells, whereas deletion of Tor1 or Ssp2 did not. Growth defects and monopolar spindle formation in a kinesin-5 mutant, cut7-446, was partially rescued by pka1 deletion or glucose limitation. These findings suggest that Pka1 and glucose limitation regulate proper spindle formation in Mal3-overexpressing cells and the cut7-446 mutant. |
| Databáze: | OpenAIRE |
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