Construction of cDNA library, nucleotide sequence and analysis of entire genome of classical swine fever virus strain Shimen

Autor: Qianhua Huang, Chuyu Zhang, Jianshi Yu, Hui Xu, Liezhen Fu, Pengwei Zhang, Jiafu Wang, Yan Zhu, Ning Wang, Jisen Huai
Rok vydání: 2000
Předmět:
Zdroj: Chinese Science Bulletin. 45:367-369
ISSN: 1861-9541
1001-6538
DOI: 10.1007/bf02909771
Popis: According to the previously published CSFV sequences, 18 pairs of primers have been designed and synthesized, which cover the entire genome of CSFV strain Shimen. Each cDNA fragment has been amplified by RT-PCR from the anticoagulant blood of strain Shimen infected pig. The PCR products have been cloned respectively and sequenced. Results show that the cDNA library of strain Shimen and its nucleotide sequence have been obtained. The genomic RNA of strain Shimen is 12 298 nucleotides in length, containing a 5′ and a 3′ noncoding region 373 and 231 nt long respectively. The center of genome is a single large open reading frame of 11 697 nt which encodes a polyprotein of 3 898 amino acids. The entire sequence of strain Shimen has also been compared with that of other CSFV strains.
Databáze: OpenAIRE
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