| Popis: |
The object ives of this study were to investigate the effect of growth regulators in callus induction, increase of biomass and to the yield more of cucurbitacin and cucurbitacin-E in leaf explants of Trichosanthes cucumerina L. var. cu- cumerina. The explants were cultured on Murashige and Skoog (M S) mediu m supplemented with different concentrations and combinations of au xins like 2,4-dich lorophenoxy acetic acid (2,4-D), (alpha)-naphthalene acetic acid (NAA), Indole butyric acid (IBA), Indole acetic acid (IAA), cytokinins like Kinetin (kn) and Ben zyl adenine (BA). The different concen- trations and combinations of BA P + IBA, 2, 4-D + BAP and 2, 4-D + kn increased the callus of fresh weight and dry weight. Among all concentrations and combinations, the best results revealed that leaf-derived callus cultured on 2,4-D (3.0mg -l ) + kin (1.0 mg -l ) produced the highest total cucurbitacins content with an optimu m y ield 4.9% w/w and cucurbitacin-E 2.75% |
