Counter-current chromatography of diaziquone by a horizontal flow-through coil planet centrifuge

Autor:	Hiroyuki Nakazawa, Rosalind D. Friedman, Feng-te Edward Chou, Yoichiroito, Nicholas R. Bachur
Rok vydání:	1982
Předmět:	Centrifuge Diaziquone Aqueous solution Chromatography Organic Chemistry General Medicine Mass spectrometry Biochemistry Analytical Chemistry Dichloroethane chemistry.chemical_compound Hydrolysis Countercurrent chromatography chemistry Methanol
Zdroj:	Journal of Chromatography A. 252:283-287
ISSN:	0021-9673
DOI:	10.1016/s0021-9673(01)88418-1
Popis:	Diaziquone is an active antitumor agent in animals that is unstable in aqueous solution. The chemical hydrolysis products of this antitumor agent have been identified and we have investigated the usefulness of the coil planet centrifuge in separating these products from the parent compound. We were able to separate the reactive diaziquone from the two reaction products using a solvent system containing dichloroethane, methanol, and sodium phosphate buffer. The identities of the products separated on the coil planet centrifuge were confirmed by thin-layer chromatography and mass spectrometry. This technique may be useful in separating metabolites contained in specimens obtained from diaziquone-treated patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::67c61d480c57d46be021df41041c4f71 https://doi.org/10.1016/s0021-9673(01)88418-1 Zobrazit plný text záznamu