The In Vitro Inhibitory Effect on Thrombin by 2,3-Diphosphoglycerate

Autor:	Vittoria Bastianon, D. Del Principe, E V Cosmi, G Biancini, A Menichelli, C. D'Arcangelo, P M Strappini, S. Del Vecchio
Rok vydání:	1981
Předmět:	Chromatography biology Chemistry Hematology Amberlite Esterase Fibrin In vitro Thrombin Polymerization medicine biology.protein Fibrinopeptide Diphosphoglycerate circulatory and respiratory physiology medicine.drug
Zdroj:	Thrombosis and Haemostasis. 46:581-583
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1653422
Popis:	SummaryThrombin, incubated with 2,3-diphosphoglycerate (150 nmol 2,3-DPG/1 NIH thrombin unit) lost up to 70% of its clotting activity, whereas the esterase activity remained unchanged. No fibrinopeptide release by thrombin was observed in the presence of 2,3-DPG. The fibrin polymerization was normal. By chromatography on Amberlite IRC-50, α-thrombin was eluted at pH 8.0. In presence of 2,3-DPG, α-thrombin was not eluted. Likely, 2,3-DPG can interfere with thrombin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::67c32fdb27a4ea92e3aee60b50b3d02e https://doi.org/10.1055/s-0038-1653422 Zobrazit plný text záznamu