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Laser Microdissection and Pressure Catapulting (LMPC) is a well-known non-contact method to isolate and collect specific cells from complex animal or plant tissues or living cultured cells. Thus, it is enabling pure and homogeneous sample preparation resulting in an eminent increase in the specificity of versatile downstream molecular analyses, like e.g., different PCR, nucleic acid and protein microarray analysis. An important innovation is the integration of computer-assisted recognition methods by object-oriented image processing. Here we show the automated recognition of sample material by applying object-oriented image processing on parameters like, e.g., density, area, neighborhood and shape. Coupled with this kind of software modules, the LMPC system is able to detect, isolate and finally capture the specimen of interest in a fully automated manner. Differential analysis of individual tissues and single cells will eliminate the averaging effect and allow the discovery of detailed differences between various cell types. |
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